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Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
Published on: April 19, 2015
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Nanofibrous scaffolds with biomimetic structure
Shahla Khalili1,2,3, Saied Nouri Khorasani1, Mohammad Razavi4,5
1Department of Chemical Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Journal of Biomedical Materials Research. Part A
|September 26, 2017
Summary
Using a grid-patterned collector significantly enhanced electrospun gelatin/cellulose acetate/elastin scaffolds. This improved pore size, mechanical properties, and fibroblast cell attachment, offering a superior microenvironment for tissue engineering applications.
Area of Science:
- Biomaterials Engineering
- Tissue Engineering
- Materials Science
Background:
- Electrospun scaffolds are crucial for tissue regeneration.
- Controlling scaffold architecture, such as pore size and fiber diameter, is vital for cellular response.
- Conventional fabrication methods may limit the ability to precisely engineer scaffold microenvironments.
Purpose of the Study:
- To investigate the impact of a grid-like patterned collector on the properties of electrospun gelatin/cellulose acetate/elastin scaffolds.
- To evaluate the effect of the patterned collector on scaffold morphology, swelling, degradation, mechanical strength, and cellular compatibility.
- To compare the performance of patterned scaffolds with those fabricated using conventional flat sheet collectors.
Main Methods:
- Fabrication of gelatin/cellulose acetate/elastin scaffolds using electrospinning with a novel grid-like patterned collector.
- Morphological analysis using scanning electron microscopy to assess pore size and fiber diameter.
- Swelling ratio and degradation rate measurements.
- Tensile testing to determine mechanical properties, specifically elongation at break.
- In vitro cell culture studies using fibroblast cells to evaluate attachment and proliferation.
Main Results:
- The grid-patterned collector resulted in enlarged pore sizes and reduced fiber diameters compared to conventional collectors.
- Scaffolds fabricated with the patterned collector exhibited increased swelling ratios and degradation rates.
- Tensile testing showed a significant increase in elongation at break, up to 145%, for the patterned scaffolds.
- In vitro studies demonstrated enhanced attachment and proliferation of fibroblast cells on the patterned scaffolds.
Conclusions:
- The grid-like patterned collector effectively modifies the architecture of electrospun gelatin/cellulose acetate/elastin scaffolds.
- The enhanced scaffold properties, including improved mechanical strength and cellular compatibility, make them promising for tissue engineering applications.
- Patterned scaffolds provide a superior microenvironment for fibroblast cells compared to conventionally fabricated scaffolds.
