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Updated: Feb 22, 2026

Author Spotlight: Unraveling the Interplay Between Trichoderma stromaticum and the Mammalian Immune System
Published on: October 20, 2023
Harzianone Biosynthesis by the Biocontrol Fungus Trichoderma
Lena Barra1, Jeroen S Dickschat1
1Kekulé-Institut für Organische Chemie und Biochemie, Rheinische Friedrich-Wilhelms-Universität Bonn, Gerhard-Domagk-Strasse 1, 53121, Bonn, Germany.
Abstract:
Analysis of the volatile terpenes produced by seven fungal strains of the genus Trichoderma by use of a closed-loop stripping apparatus (CLSA) revealed a common production of harzianone, a bioactive, structurally unique diterpenoid consisting of a fused tetracyclic 4,7,5,6-membered ring system. The terpene cyclization mechanism was studied by feeding experiments using selectively 13 C- and 2 H-labeled synthetic mevalonolactone isotopologues, followed by analysis of the incorporation patterns by 13 C NMR spectroscopy and GC/MS. The structure of harzianone was further supported from a 13 C,13 C COSY experiment of the in-vivo-generated fully 13 C-labeled diterpene.
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