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Analysis of adenovirus type 2 L1 RNA 3'-end formation in vivo and in vitro
K H Hales1, J M Birk, M J Imperiale
1Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.
Journal of Virology
|April 1, 1988
Abstract:
Downstream sequence requirements for efficient cleavage and polyadenylation at the adenovirus type 2 L1 poly(A) site were determined in vivo in 293 cells and in vitro by using RNA precursors in HeLa cell nuclear extracts. The two cleavage sites used were found to differ in sensitivity to 3'-end deletion in vivo and in vitro.