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Updated: Feb 22, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
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CMIP is oncogenic in human gastric cancer cells

Jianlin Zhang1, Jin Huang2, Xingyu Wang1

  • 1Department of Emergency Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.

Molecular Medicine Reports
|September 26, 2017
PubMed

Insights

c-Maf inducing protein (CMIP) drives gastric cancer growth and spread. Lowering CMIP levels inhibited cancer cell proliferation and migration, suggesting CMIP as a potential therapeutic target for gastric cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Gastric cancer is a leading cause of cancer mortality worldwide.
  • Recurrence, metastasis, and drug resistance impede survival in advanced gastric cancer.
  • Understanding molecular mechanisms is crucial for improving gastric cancer therapeutics.

Purpose of the Study:

  • To investigate the role of c-Maf inducing protein (CMIP) in human gastric cancer.
  • To explore the relationship between CMIP expression and clinical parameters, survival, and cellular behavior.
  • To elucidate the molecular pathways involving CMIP in gastric cancer.

Main Methods:

  • Immunohistochemistry and western blot analysis to assess CMIP protein levels.
  • In vitro assays (cell counting, CCK-8, colony formation, wound healing, Transwell) to evaluate CMIP's effect on cell proliferation and migration.
  • RNA interference (RNAi) for CMIP depletion.
  • Luciferase reporter assay and RT-qPCR to analyze upstream and downstream gene regulation.

Main Results:

  • CMIP protein expression was significantly elevated in gastric cancer tissues compared to normal tissues.
  • Higher CMIP expression correlated with poorer clinical parameters, relapse-free survival, and overall survival.
  • CMIP depletion reduced gastric cancer cell proliferation and migration in vitro.
  • CMIP was identified as a direct target of miR-101-3p.
  • CMIP knockdown led to downregulation of MDM2 and MAPK mRNA expression.

Conclusions:

  • CMIP exhibits an oncogenic role in human gastric cancer.
  • CMIP, miR-101-3p, MDM2, and MAPK are involved in a critical signaling pathway in gastric cancer.
  • CMIP represents a potential novel therapeutic target for gastric cancer management.

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