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Related Concept Videos

Thermosensation01:43

Thermosensation

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Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
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Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

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Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
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Aquaporins01:25

Aquaporins

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Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
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ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

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V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
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Reabsorption and Secretion in the DCT and Collecting Duct01:26

Reabsorption and Secretion in the DCT and Collecting Duct

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The early phase of the DCT manages the reabsorption of approximately 10-15% of filtered water, 5–10% of filtered sodium, and 5–10% of filtered chloride. This process is facilitated by Na+–Cl− symporters in apical membranes and sodium-potassium pumps, as well as Cl− leakage channels in basolateral membranes. The early DCT also stands out as a site where parathyroid hormone (PTH) stimulates calcium reabsorption, depending on the body's requirements.
The distal...
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Reabsorption and Secretion in the PCT01:28

Reabsorption and Secretion in the PCT

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The Proximal Convoluted Tubule, or PCT, plays a pivotal role in the body's filtration system. They are primarily responsible for reabsorbing solutes and water from the filtered fluid produced by the glomeruli. Most of the filtered water, ions, and organic solutes such as glucose and amino acids are reabsorbed by the PCT.
Transport mechanisms involving sodium ions (Na+) contribute significantly to solute reabsorption. These mechanisms include symport and antiport processes.
A key example is the...
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Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
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Human podocytes express functional thermosensitive TRPV channels.

Lídia Ambrus1, Balázs Kelemen1, Tamás Szabó2

  • 1DE-MTA 'Lendület' Cellular Physiology Research Group, Department of Physiology, Medical Faculty, University of Debrecen, Debrecen, Hungary.

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Heat-sensitive TRPV channels, including TRPV4, are functionally present in human podocytes. This suggests their potential role in regulating the glomerular filtration barrier and kidney function.

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A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
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Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
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Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
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Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

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Area of Science:

  • Nephrology
  • Cell Biology
  • Ion Channel Physiology

Background:

  • Transient Receptor Potential Vanilloid (TRPV) channels regulate barrier functions in epithelial tissues.
  • TRPV channel expression is known in the distal nephron but uncharacterized in podocytes.
  • Podocytes are crucial for the glomerular filtration barrier.

Purpose of the Study:

  • To investigate the presence and function of Ca2+-permeable TRPV1-4 channels in human podocytes.
  • To determine the role of these channels in podocyte function.

Main Methods:

  • Investigated TRPV1-4 expression at protein and mRNA levels in human podocytes.
  • Assessed channel function via intracellular Ca2+ measurements and patch clamp electrophysiology.
  • Utilized specific agonists and inhibitors for TRPV channel subtypes.

Main Results:

  • Thermosensitive TRPV channels are expressed in human podocytes.
  • TRPV4 agonists induced significant Ca2+ influxes, blocked by TRPV4 inhibitors.
  • TRPV2 and TRPV4 activators showed moderate Ca2+ influxes, while TRPV3 agonists had complex effects.

Conclusions:

  • Human podocytes functionally express TRPV4 and other thermosensitive TRPV channels.
  • These findings suggest a potential role for TRPV channels in regulating the glomerular filtration barrier.