Related Experiment Video
Updated: Feb 22, 2026

Bergmeyer Glucose Quantification for Microbiological Samples
Published on: January 17, 2025
Improvement in the quantification of reducing sugars by miniaturizing the Somogyi-Nelson assay using a microtiter
Yijing Shao1, Amy Hui-Mei Lin1
1Bi-State School of Food Science, University of Idaho and Washington State University, 875 Perimeter Dr. MS 2312, Moscow, ID 83843-2312, USA.
Abstract:
Measuring reducing sugar is a common practice in carbohydrate research, and the colorimetric assay developed by Somogyi and Nelson has a high sensitivity in a broad concentration range. However, the method is time-consuming when analyzing a large number of samples. In this study, a modified Somogyi-Nelson assay with excellent accuracy and sensitivity was developed using a 96-well microplate. This microassay greatly improves the analytic capacity and efficacy of the method.

