Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inositol lipids and DNA replication.

M J Berridge1

  • 1Department of Zoology, University of Cambridge, U.K.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|December 15, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Remodelling Ca2+ signalling systems and cardiac hypertrophy.

Biochemical Society transactions·2006
Same author

Cardiac calcium signalling.

Biochemical Society transactions·2003
Same author

Calcium puffs are generic InsP(3)-activated elementary calcium signals and are downregulated by prolonged hormonal stimulation to inhibit cellular calcium responses.

Journal of cell science·2001
Same author

The versatility and complexity of calcium signalling.

Novartis Foundation symposium·2001
Same author

The organisation and functions of local Ca(2+) signals.

Journal of cell science·2001
Same author

The versatility and universality of calcium signalling.

Nature reviews. Molecular cell biology·2001
Same journal

The microlandscapes of tree trunks: the effect of lichen and tree-level characteristics on arthropod communities.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same journal

Centimetre-scale landscapes to assess the motion behaviour and cognition of gastropods and bivalves.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same journal

Intertidal microcosms of wave-swept rocky shores: ecological and physiological insights from a uniquely stressful environment.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same journal

Temporal and spatial variation in temperature and oxygen at the microscale: key niche axes for aquatic life.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same journal

Natural microcosms in ecology: fulfilling the promise of model systems?

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same journal

Microbe-induced galls and plant defence: metabolite crosstalk in a co-evolutionary battle.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
See all related articles

Growth factors trigger DNA synthesis via intracellular signals like calcium and pH changes. These signals activate early genes and protein synthesis, initiating cell growth and division.

Area of Science:

  • Cellular Biology
  • Molecular Signaling

Background:

  • Growth factors control DNA synthesis through intracellular mitogenic signals.
  • Tyrosine kinase activity and changes in calcium and hydrogen ion concentrations are key events.
  • Hydrolysis of inositol lipids generates important second messengers.

Purpose of the Study:

  • To elucidate the role of intracellular signals in growth factor-mediated DNA synthesis.
  • To identify key second messengers and their downstream effects.
  • To understand the convergence of these signals on cell growth initiation.

Main Methods:

  • Investigated the role of tyrosine phosphorylation in signal transduction.
  • Analyzed changes in intracellular calcium and pH.
  • Examined the metabolism and function of inositol phosphates, including inositol 1,4,5-trisphosphate (Ins1,4,5P3) and inositol 1,3,4,5-tetrakisphosphate (Ins1,3,4,5P4).

Related Experiment Videos

Main Results:

  • Growth factors stimulate inositol lipid hydrolysis, producing diacylglycerol and Ins1,4,5P3.
  • Ins1,4,5P3 releases intracellular calcium, while Ins1,3,4,5P4 may regulate calcium entry.
  • Increased intracellular calcium and pH activate early gene transcription (e.g., fos) and S6 kinase, promoting protein synthesis.

Conclusions:

  • Intracellular calcium and pH are critical signals initiating cell growth.
  • These signals converge to regulate early gene expression and protein synthesis, preceding DNA synthesis.
  • Understanding these pathways is crucial for comprehending cell division control.