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Extract from mango mistletoes Dendrophthoe pentandra ameliorates TNBS-induced colitis by regulating CD4+ T cells in
Agustina Tri Endharti1,2, Sofy Permana3
1Department of Parasitology, Faculty of Medicine, Brawijaya University, Veteran Road, Malang, East Java, 65145, Indonesia. tinapermana@yahoo.com.
Background:
Mango mistletoes Dendrophthoe pentandra (MMDP) extract has attracted interest due to its pharmacological properties, including gastro protective effects. The aim of this study was to investigate whether MMDP extract could increase Foxp3 regulatory T cells and inhibits development of Th17 cells.
Methods:
Colitis was induced in Balb/c mice by rectal administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS). The mice were randomly divided into five groups comprising group1 receiving vehicle (the negative control), group 2-5 receiving TNBS, group 3-5 orally receiving either MMDP extract 150, 300 and 600 mg/kgBW for 7 days after TNBS administration. On day 8 of the experiment, the colon tissues were removed for histological examination, cytokine and myeloperoxidase (MPO) measurement. T-cells sub-population in mesenteric lymph nodes were analyzed by flow cytometer.
Results:
MMDP extract potently suppressed colon shortening and MPO in mice with TNBS-induced colitis. Administration of the extract significantly decreased the severity of TNBS-induced colitis in a dose-dependent manner. The extract significantly attenuated the loss of body weight (p < 0.05). These effects were associated with a remarkable amelioration of the disruption of the colonic architecture, significant reduction of the colonic MPO (p < 0.05). The extract lowered the levels of Th17-associated cytokines but increased the production of Treg-associated cytokines in mesenteric lymph node cells.
Conclusion:
Our results suggest that MMDP has the therapeutic potential to ameliorate TNBS-induced colitis symptoms revealed by histological change and inhibit IL-17 production.
Insights
Mango mistletoe extract (MMDP) shows therapeutic potential for colitis. It reduced inflammation, improved gut health, and modulated immune cells, suggesting a novel treatment for inflammatory bowel disease.
Area of Science:
- Immunology
- Pharmacology
- Gastroenterology
Background:
- Mango mistletoe Dendrophthoe pentandra (MMDP) extract exhibits gastroprotective properties.
- Research interest in MMDP extract is growing due to its potential pharmacological benefits.
Purpose of the Study:
- To investigate the therapeutic potential of MMDP extract in TNBS-induced colitis.
- To determine if MMDP extract modulates regulatory T cells (Tregs) and Th17 cells.
Main Methods:
- Colitis was induced in mice using 2,4,6-trinitrobenzene sulfonic acid (TNBS).
- Mice received varying doses of MMDP extract or vehicle control.
- Colon tissues and mesenteric lymph nodes were analyzed for histological changes, cytokine levels, MPO activity, and T-cell populations.
Main Results:
- MMDP extract significantly reduced colon shortening and myeloperoxidase (MPO) levels in TNBS-induced colitis.
- The extract attenuated body weight loss and improved colonic architecture in a dose-dependent manner.
- MMDP extract decreased Th17-associated cytokines while increasing Treg-associated cytokines in mesenteric lymph node cells.
Conclusions:
- MMDP extract demonstrates therapeutic potential for ameliorating TNBS-induced colitis.
- The extract's efficacy is linked to its ability to modulate Th17 and Treg cell populations.
- MMDP extract may offer a novel approach for managing inflammatory bowel disease.
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