Related Experiment Video
Updated: Feb 22, 2026
10:44
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
Published on: May 5, 2023
2.0K
ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin
Lifang Li1, Xinhua Liu2, Lin He1
1Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing Key Laboratory of Protein Posttranslational Modifications and Cell Function, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China.
Nature Communications
|September 27, 2017
Summary
The zinc-finger protein ZNF516 acts as a transcription repressor, inhibiting epidermal growth factor receptor (EGFR) expression. Low ZNF516 levels correlate with aggressive breast cancer, suggesting ZNF516 as a potential therapeutic target.
Area of Science:
- Molecular Biology
- Oncology
- Gene Regulation
Background:
- Epidermal growth factor receptor (EGFR) dysregulation is crucial in cancer development.
- Mechanisms controlling EGFR expression are not fully understood.
Purpose of the Study:
- To investigate the role of ZNF516 in regulating EGFR expression.
- To explore ZNF516's potential as a therapeutic target in breast cancer.
Main Methods:
- Genome-wide chromatin association analysis.
- Co-immunoprecipitation to study protein complex formation.
- In vitro and in vivo assays to assess cancer cell proliferation, invasion, and tumor growth.
Main Results:
- ZNF516 functions as a transcription repressor, binding to the CtBP/LSD1/CoREST complex.
- ZNF516 represses genes involved in cell proliferation and motility, including EGFR.
- The ZNF516 complex inhibits breast cancer cell proliferation and invasion in vitro and suppresses tumor growth and metastasis in vivo.
- Low ZNF516 expression is linked to advanced breast cancer staging and poorer patient survival.
Conclusions:
- ZNF516 is a transcriptional repressor of EGFR.
- ZNF516 acts as a tumor suppressor in breast cancer.
- ZNF516 represents a potential therapeutic target for breast cancer treatment.
More Related Videos
Related Concept Videos
02:04Co-activators and Co-repressors
8.7K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.7K
02:26NF-κB-dependent Signaling Pathway
10.1K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
10.1K
01:52Eukaryotic Transcription Inhibitors
11.1K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
11.1K
03:17Inheritance of Chromatin Structures
7.7K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.7K
01:45Regulation of Nuclear Protein Sorting
3.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.4K
01:16TGF - β Signaling Pathway
10.7K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.7K