Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Synthetic Biology

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...

Neural Circuits

Neural Circuits

Neural circuits and neuronal pools are two of the main structures found in the nervous system. Neural circuits are networks of neurons that work together to carry out a specific task or process. They consist of interconnected neurons and glial cells, which provide structural and metabolic support.
Neuronal pools are collections of nerve cells with similar functions and interact through chemical and electrical signals. These pools include both interneurons (the central neural circuit nodes that...

Network Function of a Circuit

Network Function of a Circuit

Frequency response analysis in electrical circuits provides vital insights into a circuit's behavior as the frequency of the input signal changes. The transfer function, a mathematical tool, is instrumental in understanding this behavior. It defines the relationship between phasor output and input and comes in four types: voltage gain, current gain, transfer impedance, and transfer admittance. The critical components of the transfer function are the poles and zeros.

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

Protein Networks

Protein Networks

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Competitive Metabolism of Terephthalic Acid by a Consortium of Native and Engineered Bacteria.

ACS synthetic biology·2026

Same author

Intracellular acidification by microbiota-derived valeric acid facilitates trans-kingdom ecology limiting Candida parapsilosis colonization.

Cell host & microbe·2026

Same author

Gut Commensals Regulate the Intestinal Kynurenine Pathway.

bioRxiv : the preprint server for biology·2026

Same author

A microbiota-derived bile acid overcomes antibiotic-induced hyporesponsiveness to immune checkpoint therapy by enhancing CD8 <sup>+</sup> T cell antitumor immunity.

bioRxiv : the preprint server for biology·2026

Same author

Polystyrene microplastics disrupt the blood-testis barrier via CEBPB-driven lysosomal autophagy and induce ferroptosis-like injury in human sperm, compromising embryo development.

Journal of hazardous materials·2026

Same author

Single-cell and spatial transcriptomic analysis reveals PAX5's role and regulatory mechanisms in gastric adenocarcinoma lymph node metastasis.

Journal of translational medicine·2026

Search research articles

Related Experiment Video

Updated: Feb 22, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

An integrative circuit-host modelling framework for predicting synthetic gene network behaviours.

Chen Liao^1,2, Andrew E Blanchard^2,3, Ting Lu^4,5,6,7

¹Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

Nature Microbiology

|September 27, 2017

Summary

This study presents a new gene circuit modeling framework that links synthetic biology circuits with host physiology. This bidirectional coupling improves prediction of engineered gene circuit behaviors in synthetic biology.

More Related Videos

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Published on: September 13, 2024

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Related Experiment Videos

Last Updated: Feb 22, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Published on: September 13, 2024

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Area of Science:

Synthetic biology
Systems biology
Computational biology

Background:

Quantitative prediction of engineered gene circuit behavior is a major challenge in synthetic biology.
The complex interdependence between gene circuits and their host organisms contributes to this challenge.

Purpose of the Study:

To develop a modeling framework that integrates gene circuit behavior with host physiology.
To enable accurate prediction and understanding of engineered gene circuit dynamics.

Main Methods:

A mechanistic, coarse-grained host physiology model with dynamic resource partitioning.
Multilayered, bidirectional coupling between circuits and host, including generic and specific interactions.
A detailed kinetic module for exogenous circuits.

Main Results:

The framework successfully captured and predicted experimental data for host and foreign gene overexpression.
Analysis of a growth-modulating feedback circuit revealed altered dynamics due to circuit-host interactions.
An extended framework elucidated toggle switch behaviors across single-cell, population, and spatial scales.

Conclusions:

The developed framework advances quantitative understanding of gene circuit behaviors.
This approach benefits the rational design of synthetic gene networks by accounting for circuit-host interactions.