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Antimicrobial susceptibilities of Bordetella species isolated in a Multicenter Pertussis Surveillance Project
T A Kurzynski1, D M Boehm, J A Rott-Petri
1State Laboratory of Hygiene, University of Wisconsin, Madison 53706.
Abstract:
MICs for 90% (MIC90s) of 75 Bordetella pertussis strains for amoxicillin, erythromycin, rifampin, and sulfamethoxazole-trimethoprim were 1, less than or equal to 0.12, 1, and 4 micrograms/ml, respectively. Susceptibility rates were all greater than or equal to 93%. Only 17% of the strains were susceptible to tetracycline. The MIC90s of ciprofloxacin, enoxacin, norfloxacin, ofloxacin, and roxithromycin were less than or equal to 0.06, 0.5, 0.25, 0.12, and 0.5 micrograms/ml, respectively. For B. parapertussis, the MIC90s were 16-fold higher with amoxicillin and rifampin and 2- to 4-fold higher with the fluoroquinolones and roxithromycin.
Insights
This study assessed antibiotic susceptibility in Bordetella pertussis and Bordetella parapertussis. Most strains were susceptible to erythromycin and fluoroquinolones, but not tetracycline.
Area of Science:
- Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background:
- Pertussis, caused by Bordetella pertussis, remains a significant public health concern.
- Understanding antibiotic resistance patterns is crucial for effective treatment and control of pertussis outbreaks.
Purpose of the Study:
- To determine the in vitro antimicrobial susceptibility of Bordetella pertussis and Bordetella parapertussis strains.
- To evaluate the efficacy of various antibiotics against these key Bordetella species.
Main Methods:
- Minimum Inhibitory Concentrations (MICs) were determined for multiple antibiotics against 75 Bordetella pertussis strains.
- Susceptibility testing included amoxicillin, erythromycin, rifampin, sulfamethoxazole-trimethoprim, tetracycline, and several fluoroquinolones.
Main Results:
- Bordetella pertussis strains showed high susceptibility (>93%) to erythromycin and fluoroquinolones (ciprofloxacin, ofloxacin, etc.).
- Low susceptibility (<17%) was observed for tetracycline.
- Bordetella parapertussis strains exhibited significantly higher MICs for amoxicillin, rifampin, and fluoroquinolones compared to B. pertussis.
Conclusions:
- Erythromycin and fluoroquinolones are effective against the studied Bordetella pertussis strains.
- Resistance to tetracycline is prevalent in Bordetella pertussis.
- Bordetella parapertussis demonstrates reduced susceptibility to several key antibiotics compared to Bordetella pertussis.