DNA Microcapsule for Photo-Triggered Drug Release Systems
Yukiko Kamiya1,2, Yoshinobu Yamada1, Takahiro Muro1
1Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
Chemmedchem
|September 27, 2017
Summary
Researchers developed light-responsive DNA microcapsules using photo-switchable DNA strands. These biocompatible capsules enable controlled release of drugs like doxorubicin, showing potential for targeted therapies.
Area of Science:
- Biotechnology
- Materials Science
- Nanotechnology
Background:
- Development of responsive materials for controlled substance delivery is crucial.
- DNA self-assembly offers a versatile platform for creating complex nanostructures.
- Photo-responsive components are needed for external control of material properties.
Purpose of the Study:
- To construct spherical, photo-responsive microcapsules using DNA self-assembly.
- To investigate the light-induced deformation and drug release capabilities of these microcapsules.
- To evaluate the potential of these microcapsules as a photo-controlled drug-release system.
Main Methods:
- Fabrication of microcapsules using three photo-switchable DNA strands forming a three-way junction (TWJ) motif.
- Incorporation of azobenzene (Azo) or SDM-Azo photo-switches into DNA sticky-end regions via d-threoninol linkers.
- Induction of structural deformation and small molecule release using specific light irradiation.
- Assessment of doxorubicin release and its cytotoxicity on cultured cells.
Main Results:
- Successfully constructed spherical DNA microcapsules with photo-responsive properties.
- Demonstrated light-induced deformation of the microcapsule structure via azobenzene isomerization.
- Achieved photo-triggered release of encapsulated small molecules, including doxorubicin.
- Showcased doxorubicin-induced cytotoxicity in cultured cells, confirming effective drug delivery.
Conclusions:
- The developed DNA microcapsules are biocompatible and exhibit photo-responsive behavior.
- These microcapsules can be utilized for light-controlled release of encapsulated drugs.
- The system holds significant potential for advanced photo-controlled drug delivery applications.
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