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2H1--a novel antigen involved in T lymphocyte triggering
C Morimoto1, C E Rudd, N L Letvin
1Dana-Farber Cancer Institute, Department of Medicine, Boston, MA 02115.
Journal of Immunology (Baltimore, Md. : 1950)
|April 1, 1988
Summary
A new antibody, anti-2H1, targets a specific antigen (2H1 Ag) on activated T lymphocytes. This discovery offers potential for understanding and modulating T cell activation in immune responses.
Area of Science:
- Immunology
- Cell Biology
Background:
- T lymphocytes play a crucial role in adaptive immunity.
- Identifying specific cell surface markers is vital for understanding T cell function and activation.
Purpose of the Study:
- To produce and characterize a monoclonal antibody (mAb) against a novel antigen on activated T cells.
- To investigate the role of the 2H1 antigen in T lymphocyte activation.
Main Methods:
- Production of a mAb (anti-2H1) against PHA-activated T cells from *Aotus trivirgatus*.
- Flow cytometry to determine antigen expression on various immune cell populations.
- Analysis of T cell activation and proliferation induced by anti-2H1.
- SDS-PAGE to identify the molecular weight of the 2H1 antigen.
Main Results:
- Anti-2H1 reacted with 90% of peripheral T cells and activated thymocytes, but not B cells or macrophages.
- The 2H1 antigen expression increased upon T cell activation with Con A plus PMA.
- Anti-2H1 antibody induced T cell activation and proliferation, particularly in synergy with PMA or anti-T11(3).
- SDS-PAGE revealed two major polypeptides (140 and 105 kDa) for the 2H1 antigen, distinguishing it from T3, T11, and 9.3 antigens.
Conclusions:
- The 2H1 antigen is a novel marker expressed on activated T lymphocytes.
- The 2H1 antigen is involved in T lymphocyte activation and proliferation.
- The anti-2H1 antibody represents a valuable tool for studying T cell activation pathways.