New 12,8-Eudesmanolides from Eutypella sp. 1-15.

Area of Science:

• Natural Products Chemistry
• Medicinal Chemistry
• Mycology

Background:

• Mangrove ecosystems harbor diverse microorganisms with potential for novel compound discovery.
• Fungal secondary metabolites, such as eudesmanolides, are known for their biological activities.
• Eutypella species are recognized sources of bioactive natural products.

Purpose of the Study:

• To isolate and characterize new eudesmanolides from a mangrove-derived fungus.
• To determine the structures and absolute stereochemistry of the isolated compounds.
• To evaluate the anticancer and antimicrobial potential of the isolated compounds.

Main Methods:

• Isolation and purification of compounds using chromatographic techniques.
• Structure elucidation through comprehensive spectroscopic data (NMR, MS).
• Determination of absolute stereochemistry via experimental and calculated Electronic Circular Dichroism (ECD) analysis.
• In vitro anticancer activity assay against JEKO-1 and HepG2 cell lines.
• Antimicrobial activity screening.

Main Results:

• Four new 12,8-eudesmanolides (1-4) and one known compound (5) were isolated from Eutypella sp. 1-15.
• The structures and absolute stereochemistry of compounds 1-4 were fully elucidated.
• Compound 1 demonstrated potent anticancer activity against JEKO-1 (IC50 = 8.4 μM) and HepG2 (IC50 = 28.5 μM) cell lines.
• Compound 1 also exhibited moderate antimicrobial activity.

Conclusions:

• The study successfully identified novel eudesmanolides from a mangrove fungus.
• Compound 1 possesses significant anticancer and moderate antimicrobial properties, suggesting its therapeutic potential.
• This research contributes to the understanding of chemical diversity in mangrove-associated fungi and their bioactive metabolites.