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Updated: Feb 22, 2026

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A Novel Cellulase Produced by a Newly Isolated Trichoderma virens.

Rong Zeng1, Xiao-Yan Yin2, Tao Ruan3

  • 1College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China. rongzengce@163.com.

Bioengineering (Basel, Switzerland)
|September 28, 2017
PubMed
Summary

Researchers isolated a novel fungus, Trichoderma virens ZY-01, capable of producing high-activity cellulase enzyme. This discovery offers a promising avenue for improving crop straw utilization through enhanced enzyme production.

Keywords:
Trichoderma virensbiomasscellulasestrain isolationstraw

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Area of Science:

  • Biotechnology
  • Enzymology
  • Microbiology

Background:

  • Improving crop straw utilization is crucial for sustainable agriculture.
  • High-activity cellulase enzymes are key to efficient biomass conversion.
  • Novel microbial strains are needed for enhanced enzyme production.

Purpose of the Study:

  • To isolate and characterize a novel microbial strain producing high-activity cellulase.
  • To optimize cellulase production and purification processes.
  • To investigate the enzymatic properties of the purified cellulase.

Main Methods:

  • Isolation of microbial strains using soil dilution plate method with cellulose and Congo red.
  • Fermentation of the novel strain Trichoderma virens ZY-01.
  • Purification of cellulase via (NH₄)₂SO₄ fractional precipitation, anion-exchange, and gel filtration chromatography.
  • Enzymatic characterization including optimum temperature, pH, and metal ion effects.

Main Results:

  • A novel strain, Trichoderma virens ZY-01, was successfully isolated and patented.
  • Cellulase activity reached 7.4 IU/mL under non-optimized fermentation.
  • Purification yielded a 35.8-fold increase in specific activity (31.5 IU/mg) with 47.04% yield.
  • Optimum activity at 50 °C and pH 5.0; enhanced by Zn²⁺, Ca²⁺, Mn²⁺; inhibited by Cu²⁺, Co²⁺.

Conclusions:

  • The novel Trichoderma virens ZY-01 strain is a highly efficient producer of cellulase.
  • The purified cellulase exhibits favorable enzymatic properties for potential industrial applications.
  • This research contributes a valuable resource for crop straw utilization and biofuel production.