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Published on: June 17, 2015
Assaying RNA Localization in Situ with Spatially Restricted Nucleobase Oxidation
Ying Li1, Mahima B Aggarwal1, Kim Nguyen1
1Department of Pharmaceutical Sciences and ‡Department of Chemistry, University of California, Irvine , Irvine, California 92697, United States.
Abstract:
We report herein a novel chemical-genetic method for assaying RNA localization within living cells. RNA localization is critical for normal physiology as well as the onset of cancer and neurodegenerative disorders. Despite its importance, there is a real lack of chemical methods to directly assay RNA localization with high resolution in living cells. Our novel approach relies on in situ nucleobase oxidation by singlet oxygen generated from spatially confined fluorophores. We demonstrate that our novel method can identify RNA molecules localized within specific cellular compartments. We anticipate that this platform will provide the community with a much-needed methodology for tracking RNA localization within living cells, and set the stage for systematic large scale analysis of RNA localization in living systems.
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