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Related Experiment Video

Updated: Feb 22, 2026

Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
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Localized surface plasmon resonance-based abscisic acid biosensor using aptamer-functionalized gold nanoparticles.

Shun Wang1,2, Wei Li2,3, Keke Chang1,2

  • 1College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, China.

Plos One
|September 28, 2017
PubMed
Summary

A new biosensor detects abscisic acid (ABA), a key plant hormone, using aptamer-functionalized gold nanoparticles and localized surface plasmon resonance (LSPR). This method offers a simple, sensitive, and antibody-free approach for plant hormone analysis.

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Area of Science:

  • Plant Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Abscisic acid (ABA) is crucial for plant abiotic stress responses and signaling.
  • Accurate ABA quantification is essential for understanding plant physiology.
  • Existing detection methods can be costly or complex.

Purpose of the Study:

  • To develop a novel, cost-effective, and sensitive method for quantifying abscisic acid (ABA).
  • To utilize aptamer-functionalized gold nanoparticles (AuNPs) and localized surface plasmon resonance (LSPR) for ABA detection.
  • To demonstrate the method's applicability in real plant samples.

Main Methods:

  • Aptamer-functionalized gold nanoparticles (AuNPs) were synthesized.
  • ABA binding to aptamers induced changes in AuNPs' stability and aggregation.
  • Localized surface plasmon resonance (LSPR) spectra were monitored to detect ABA concentration.
  • The method was validated using rice leaf extracts and compared to ELISA.

Main Results:

  • The LSPR-based method demonstrated a linear detection range from 5×10⁻⁷ M to 5×10⁻⁵ M for ABA.
  • A low detection limit of 0.33 μM was achieved under optimized conditions.
  • The biosensor showed high selectivity and sensitivity, with relative errors between 6.59% and 7.93% compared to ELISA for rice leaf samples.

Conclusions:

  • A simple, selective, and sensitive LSPR-based biosensor for ABA detection was successfully developed.
  • The method avoids the need for expensive instruments and antibodies.
  • This aptamer-based LSPR platform holds potential for detecting other plant hormones.