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Updated: Feb 22, 2026
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
Characterisation of complete high- and low-risk human papillomavirus genomes isolated from cervical specimens in
Gisele R de Oliveira1,2, Juliana D Siqueira2, Fabiana Finger-Jardim1
1Universidade Federal do Rio Grande, Escola de Medicina, Laboratório de Biologia Molecular, Rio Grande, RS, Brasil.
Abstract:
The classification of human papillomavirus (HPV) intratypic lineages by complete genome sequencing is a determinant in understanding biological differences in association with this disease. In this work, we have characterised complete HPV genomes from southern Brazil. Fifteen cervicovaginal Pap smear negative samples previously categorised as HPV-positive were sequenced using ultradeep sequencing, and 18 complete genomes from 13 different HPV types were assembled. Phylogenetic and genetic distance analyses were performed to classify the HPV genomes into lineages and sublineages. This is the first report describing the distribution of HPV intratype lineages of high and low oncogenic risk in asymptomatic women from southern Brazil.
