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Updated: Feb 22, 2026
Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes
Published on: August 31, 2022
Cacipacore virus as an emergent mosquito-borne Flavivirus
Mario Luis Garcia de Figueiredo1,2, Alberto Anastacio Amarilla1, Glauciane Garcia de Figueiredo1
1Laboratório de Virologia, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Introduction::
Cacipacore virus (CPCV), a possible bird-associated flavivirus, has yet to be detected in mosquitoes. Our purpose is examining CPCV in mosquitoes from the Amazon region of Brazil.
Methods::
Approximately 3,253 Culicidae (grouped into 264 pools) were collected from the Amazon region during 2002-2006 and analyzed using a Flavivirus genus-specific reverse transcription- polymerase chain reaction followed by nested polymerase chain reaction assay and by nucleotide sequencing of amplicons.
Results::
Nucleotide sequences from five mosquito samples showed high similarity to the those of CPCV originally isolated in the Amazon region.
Conclusions::
This is the first report of CPCV-infected mosquitoes which has implications on the arbovirus maintenance in nature and transmission to man.
