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Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Published on: May 29, 2011
A Fluorescent Coumarin-Based Probe for the Fast Detection of Cysteine with Live Cell Application
Rui-Feng Zeng1, Jin-Shuai Lan2, Xiao-Die Li3
1School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. ZRF0049@163.com.
Abstract:
A new coumarin-based fluorescent probe, containing an allylic esters group, has been designed and synthesized for sensing cysteine in physiological pH. In this fluorescent probe, the coumarin was applied as the fluorophore and an allylic esters group was combined as both a fluorescence quencher and a recognition unit. The probe can selectively and sensitively detect cysteine (Cys) over homocysteine, glutathione, and other amino acids, and has a rapid response time of 30 min and a low detection limit of 47.7 nM. In addition, the probe could be applied for cell imaging with low cytotoxicity.
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