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Related Concept Videos

Analysis of Human Natural Killer Cell Metabolism09:03

Analysis of Human Natural Killer Cell Metabolism

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In this paper, we describe a method to measure glycolysis and mitochondrial respiration in primary human Natural Killer (NK) cells isolated from peripheral blood, at rest or following IL15-induced activation. The protocol described could be easily extended to primary human NK cells activated by other cytokines or soluble...
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A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro10:36

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Here, we present a protocol to mass-produce gene-silencing murine NK cells by using a feeder-free differentiation system for mechanistic study in vitro and in...
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Isolating Sensory Neurons and Co-Culturing with Natural Killer Cells03:37

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This video describes the isolation of DRG neurons through enzymatic digestion, mechanical dissociation, and gradient centrifugation. The DRG cells are co-cultured with natural killer cells on an extracellular matrix to study the interaction between immune cells and sensory...
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Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells03:14

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This video demonstrates a technique for the expansion of natural killer (NK) cells directly from peripheral blood mononuclear cells (PBMCs) using a feeder cell line. PBMCs are co-cultured with genetically modified lymphoblastoid feeder cells expressing membrane-bound interleukin-21 (mIL-21), and the culture is supplemented with the cytokines interleukin-2 (IL-2) and interleukin-15 (IL-15). Together, this cocktail of ligands leads to the prolonged expansion of NK cells with high...
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Natural Killer Cell Isolation from Liver Tissue for Selective Expansion04:17

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This video demonstrates the isolation of lymphocytes, including natural killer cells or NK cells, from human liver tissue for selective expansion. Enzymatic and mechanical digestion, followed by density gradient separation, promotes lymphocyte isolation, including NK cells. The collected lymphocytes can be further used for the selective expansion of NK...
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Isolating Invariant Natural Killer T Cells from a Mouse Model03:35

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This video illustrates a method for isolating invariant natural killer T cells, also known as iNKT cells. The procedure begins with injecting a synthetic glycolipid into a mouse to stimulate the activation and proliferation of iNKT cells in the spleen. Subsequently, single-cell suspensions are prepared from the spleen and incubated with iNKT-cell-recognizing complexes labeled with fluorophores, followed by magnetic bead-based separation, to isolate the iNKT...
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Related Experiment Video

Updated: Jan 20, 2026

Analysis of Human Natural Killer Cell Metabolism
09:03

Analysis of Human Natural Killer Cell Metabolism

Published on: June 22, 2020

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Natural Killer Cell Memory: Progress and Implications.

Hui Peng1, Zhigang Tian1,2

  • 1Institute of Immunology, The CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, China.

Frontiers in Immunology
|September 29, 2017
PubMed
Summary
This summary is machine-generated.

Natural killer (NK) cells, once thought to be short-lived, can form immunological memory. This discovery opens new avenues for harnessing NK cell memory in cancer immunotherapy.

Keywords:
contact hypersensitivitycytomegalovirus infectionimmunological memorynatural killer celltherapeutic potential

More Related Videos

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Area of Science:

  • Immunology
  • Cellular Biology
  • Cancer Research

Background:

  • Immunological memory is a hallmark of adaptive immunity.
  • Natural killer (NK) cells were traditionally viewed as innate lymphocytes with rapid, non-specific responses.
  • Recent findings challenge this view, demonstrating NK cells can develop antigen-specific memory.

Purpose of the Study:

  • To review the evidence for NK cell memory across various contexts.
  • To summarize the underlying mechanisms of NK cell memory formation.
  • To discuss the therapeutic potential of memory-like NK cells in cancer immunotherapy.

Main Methods:

  • Literature review of immunological memory in NK cells.
  • Analysis of studies on NK cell responses to haptens and viruses.
  • Examination of research on cytokine-induced memory-like NK cells.

Main Results:

  • NK cells exhibit long-lasting, antigen-specific memory to haptens and viruses.
  • Cytokine stimulation can induce NK cells with memory-like properties, including longevity and enhanced function.
  • Growing evidence supports the role of NK cell memory in adaptive immunity.

Conclusions:

  • NK cells possess memory capabilities previously attributed only to adaptive immune cells.
  • Understanding NK cell memory mechanisms is crucial for developing novel immunotherapies.
  • Harnessing memory-like NK cells holds significant promise for cancer treatment.