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Updated: Feb 22, 2026

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
Published on: April 30, 2014
Specific titin and myomesin domains stimulate myoblast proliferation
Irina V Kravchenko1, Vladimir A Furalyov1, Vladimir O Popov1
1Federal Research Centre of Biotechnology, Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskiy prospect 33, 119071 Moscow, Russia.
Abstract:
Myofibrillar proteins titin and myomesin stimulated myoblast proliferation as determined by MTT-test and labelled thymidine incorporation in the DNA. Specific Fn type III and Ig-like domains of these proteins were able to exert mitogenic effects as well. Proliferative effect of Fn type III domains was highly sensitive to inhibition of Ca2+/calmodulin dependent protein kinase, whereas the effect of Ig-like domains showed greater sensitivity to the inhibition of adenylyl cyclase - cAMP - PKA pathway. IGF-1 autocrine signalling inhibition partially suppressed mitogenic effects revealed by both domain types.
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