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PCR

PCR

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Feb 22, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

[Progress in digital PCR technology and application].

Jiaqi Lin¹, Guocheng Su^1,2, Wenjin Su^1,2

¹College of Food and Bioengineering, Jimei University, Xiamen 361021, Fujian, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology

|September 29, 2017

Summary

This summary is machine-generated.

Digital PCR offers precise DNA quantification by isolating single molecules for analysis. This advanced technology provides higher sensitivity and accuracy for various applications like gene mutation detection and genetically modified food analysis.

Keywords:

absolute quantification copy number variation detection digital PCR genetically modified food detection microbial detection mutation detection poisson distribution

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Area of Science:

Molecular Biology
Biotechnology
Analytical Chemistry

Background:

Real-time PCR (realtime-PCR) is a standard for DNA quantification.
Digital PCR (dPCR) has emerged as a more advanced technique for absolute quantification.
dPCR offers superior sensitivity and accuracy compared to traditional methods.

Purpose of the Study:

To review the quantitative methods of digital PCR.
To summarize the research progress of dPCR in key application areas.
To highlight the advantages of dPCR over existing technologies.

Main Methods:

Digital PCR involves partitioning single DNA molecules into isolated reactions.
Amplification of partitioned DNA molecules.
Detection and analysis of fluorescence signals post-amplification.

Main Results:

Digital PCR achieves absolute quantification without relying on a standard curve.
Demonstrated higher sensitivity and accuracy in DNA analysis.
Successful application in fields like next-generation sequencing, gene mutation detection, copy number variation analysis, microorganism detection, and genetically modified food analysis.

Conclusions:

Digital PCR is a rapidly developing technology with significant advantages.
Its high sensitivity and accuracy make it suitable for diverse and demanding applications.
dPCR represents a significant advancement in nucleic acid quantification.