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Cloned hst gene from normal human leukocyte DNA transforms NIH3T3 cells
H Sakamoto1, T Yoshida, M Nakakuki
1Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Biochemical and Biophysical Research Communications
|March 30, 1988
Summary
The human stomach and tela (hst) gene, identified in stomach cancers, was investigated. Rearrangements in the hst gene were found in transformed cells, suggesting a role in cancer development.
Area of Science:
- Molecular biology
- Oncology
- Genetics
Background:
- The human stomach and tela (hst) gene was initially identified as a transforming gene in stomach cancer DNA.
- The hst gene sequence from leukemia patient DNA was previously determined.
Purpose of the Study:
- To isolate and characterize cosmid clones containing the hst gene from normal human leukocyte DNA and transformed NIH3T3 cells.
- To compare the transforming efficiency and restriction maps of the hst gene from different sources.
Main Methods:
- Isolation of cosmid clones containing the hst gene.
- Transfection assays using NIH3T3 cells.
- Restriction mapping of the hst gene.
Main Results:
- Cosmid clones containing the hst gene were isolated from normal human leukocyte DNA and transformed NIH3T3 cells.
- All isolated hst gene clones demonstrated similar transforming efficiency in NIH3T3 cells.
- The hst gene from transformed cells showed a rearrangement upstream of the TATA box compared to the normal gene.
Conclusions:
- The hst gene's transforming ability is conserved across different sources.
- A specific rearrangement in the hst gene is associated with cellular transformation, potentially contributing to cancer development.