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Updated: Feb 22, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
Published on: December 2, 2009
Why Are the Correlations between mRNA and Protein Levels so Low among the 275 Predicted Protein-Coding Genes on Human
Ekaterina V Poverennaya1, Ekaterina V Ilgisonis1, Elena A Ponomarenko1
1Institute of Biomedical Chemistry RAS , 119121 Moscow, Russia.
Abstract:
In this work targeted (selected reaction monitoring, SRM, PASSEL: PASS00697) and panoramic (shotgun LC-MS/MS, PRIDE: PXD00244) mass-spectrometric methods as well as transcriptomic analysis of the same samples using RNA-Seq and PCR methods (SRA experiment IDs: SRX341198, SRX267708, SRX395473, SRX390071) were applied for quantification of chromosome 18 encoded transcripts and proteins in human liver and HepG2 cells. The obtained data was used for the estimation of quantitative mRNA-protein ratios for the 275 genes of the selected chromosome in the selected tissues. The impact of methodological limitations of existing analytical proteomic methods on gene-specific mRNA-protein ratios and possible ways of overcoming these limitations for detection of missing proteins are also discussed.
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