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Related Experiment Video

Updated: Feb 22, 2026

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
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Microarray Expression Profile and Functional Analysis of Circular RNAs in Osteosarcoma.

Weihai Liu, Jiajun Zhang, Changye Zou

    Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
    |September 29, 2017
    PubMed
    Summary

    This study identified differentially expressed circular RNAs (circRNAs) in osteosarcoma, revealing potential roles for hsa_circRNA_103801 and hsa_circRNA_104980 in tumor development. These findings suggest circRNAs could be novel biomarkers for osteosarcoma diagnosis and treatment.

    Keywords:
    Bioinformatics analysisCircular RNAsMicroarrayOsteosarcoma (OS)

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    Area of Science:

    • Oncology
    • Molecular Biology
    • Genetics

    Background:

    • Osteosarcoma (OS) is a primary bone cancer in children and adolescents with poorly understood molecular mechanisms.
    • Circular RNAs (circRNAs) are emerging regulators of gene expression, acting as microRNA sponges.
    • Investigating circRNA expression profiles in OS is crucial for understanding tumorigenesis.

    Purpose of the Study:

    • To profile global circRNA expression in osteosarcoma.
    • To identify differentially expressed circRNAs in OS.
    • To elucidate the functional roles and interactions of key circRNAs in OS progression.

    Main Methods:

    • circRNA expression profiling using microarray analysis in OS cell lines and normal controls.
    • Validation of differentially expressed circRNAs via real-time quantitative PCR (qPCR) in OS cell lines and tissues.
    • Bioinformatic prediction of circRNA/microRNA/mRNA interactions and functional pathway analysis (GO, KEGG, Biocarta, PANTHER, Reactome).

    Main Results:

    • A distinct circRNA expression profile was identified in osteosarcoma.
    • Twelve differentially expressed circRNAs were confirmed, including up-regulated hsa_circRNA_103801 and down-regulated hsa_circRNA_104980.
    • Functional analysis linked hsa_circRNA_103801 to cancer-related pathways (e.g., HIF-1, VEGF, angiogenesis, PI3K-Akt) and hsa_circRNA_104980 to the tight junction pathway.

    Conclusions:

    • This study provides the first comprehensive circRNA expression profile in osteosarcoma.
    • Predicted ceRNA networks highlight hsa_circRNA_103801 and hsa_circRNA_104980 as potentially significant in OS initiation and progression.
    • circRNAs represent promising novel biomarkers for osteosarcoma diagnosis and therapeutic strategies.