Related Experiment Video
Updated: Feb 22, 2026
05:30
Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis
Published on: November 8, 2024
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MTA3 Regulates Extravillous Trophoblast Invasion Through NuRD Complex
Ying Chen1, Sok Kean Khoo2, Richard Leach3
1Department of Biomedical Sciences, Grand Valley State University, Allendale, MI, USA.
AIMS Medical Science
|September 30, 2017
Summary
Metastasis-associated protein 3 (MTA3) regulates extravillous trophoblast (EVT) invasion and gene expression. Downregulating MTA3 increases EVT invasion and expression of matrix metalloproteinases and Snail via the NuRD complex.
Area of Science:
- Reproductive Biology
- Cell Biology
- Molecular Biology
Background:
- Extravillous trophoblast (EVT) invasion is crucial for placenta development and remodeling uterine arteries.
- Impaired EVT invasion is linked to placenta-related diseases like preeclampsia.
- Metastasis-associated protein 3 (MTA3), a component of the Nu nucleosome remodeling and deacetylation (NuRD) complex, is downregulated in preeclamptic placentas, suggesting a role in EVT invasion.
Purpose of the Study:
- To investigate the role of MTA3 in EVT invasion.
- To elucidate the molecular mechanisms by which MTA3 regulates EVT invasion.
Main Methods:
- Immunohistochemistry to confirm MTA3 expression in human placental EVT cells.
- Lentivirus-mediated short hairpin RNA (shRNA) to knockdown MTA3 in HTR8/SVneo cells.
- Quantitative real-time PCR, co-immunoprecipitation-Western blot, and co-immunoprecipitation-Mass spectrometry assays.
Main Results:
- MTA3 knockdown in HTR8/SVneo cells led to increased invasion capacity.
- Knockdown cells exhibited higher expression of invasion-related genes: matrix metalloproteinase 2 (MMP2), matrix metalloproteinase 9 (MMP9), and transcription factor Snail.
- Co-immunoprecipitation assays confirmed interactions between MTA3 and histone deacetylase 1 (HDAC1), a NuRD subunit, and identified 71 MTA3-interacting proteins, including NuRD components and epigenetic modifiers.
Conclusions:
- MTA3 plays a regulatory role in EVT invasion.
- MTA3 influences the expression of key invasion-related genes.
- MTA3 exerts its function in EVT invasion and gene regulation through the NuRD complex.
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