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Related Concept Videos

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Related Experiment Video

Updated: Feb 22, 2026

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
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Nanofiber-based sutures induce endogenous antimicrobial peptide.

Shixuan Chen1, Liangpeng Ge2, Adrian F Gombart3

  • 1Department of Surgery-Transplant & Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA.

Nanomedicine (London, England)
|September 30, 2017
PubMed
Summary

New nanofiber sutures co-loaded with 25-hydroxyvitamin D3 (25D3) and pam3CSK4 peptide can stimulate the body's own antimicrobial peptide production. This innovation shows promise for reducing surgical site infections.

Keywords:
25-hydroxyvitamin D3co-deliveryelectrospun nanofiberspam3Cys-Ser-(Lys)4 peptidesurgical site infectionsutures

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Area of Science:

  • Biomaterials Engineering
  • Wound Healing
  • Infectious Disease Research

Background:

  • Surgical site infections remain a significant clinical challenge.
  • Current antimicrobial strategies often face resistance issues.
  • Stimulating endogenous antimicrobial peptide production offers a novel therapeutic approach.

Purpose of the Study:

  • To develop advanced nanofiber sutures capable of inducing endogenous antimicrobial peptide production.
  • To create a localized delivery system for key therapeutic agents.
  • To investigate the potential of these sutures in preventing surgical site infections.

Main Methods:

  • Co-axial electrospinning and rolling techniques were employed to fabricate nanofiber sutures.
  • Sutures were loaded with 25-hydroxyvitamin D3 (25D3) and pam3CSK4 peptide.
  • In vitro studies assessed drug release profiles and cellular responses.

Main Results:

  • Nanofiber sutures exhibited tunable mechanical properties meeting USP standards.
  • Sustained release of 25D3 over 4 weeks and an initial burst release of pam3CSK4 were observed.
  • Co-delivery enhanced cathelicidin antimicrobial peptide production in U937 cells and keratinocytes.
  • Cell proliferation assays confirmed no significant cytotoxicity to relevant cell lines.

Conclusions:

  • Co-loaded 25D3/pam3CSK4 peptide nanofiber sutures effectively induce endogenous antimicrobial peptide production.
  • This technology holds potential for reducing the incidence of surgical site infections.
  • The developed sutures represent a promising biomaterial for infection control in surgical settings.