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Related Experiment Videos

Lymphocyte aggregation in response to adrenergic stimulation.

D E Hammerschmidt1, C Jeanneret, M Husak

  • 1Department of Medicine, University of Minnesota Medical School, Minneapolis.

Blood
|May 1, 1988
PubMed
Summary
This summary is machine-generated.

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Chronic lymphocytic leukemia (CLL) B cells can aggregate when stimulated by certain agents, suggesting a role for beta-adrenergic receptors in lymphocyte adhesion and potential morbidity in patients with high B cell counts.

Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • Chronic lymphocytic leukemia (CLL) is characterized by a high lymphocyte count.
  • Leukapheresis is used to reduce extreme lymphocyte counts.
  • The impact of leukapheresis on lymphocyte adhesive properties is not well understood.

Purpose of the Study:

  • To investigate the adhesive properties of chronic lymphocytic leukemia (CLL) B cells.
  • To determine if beta-adrenergic receptors are present on CLL B cells.
  • To explore the potential role of B cell aggregation in CLL-related morbidity.

Main Methods:

  • In vitro lymphocyte aggregation assays using various stimuli (arachidonic acid, calcium, epinephrine).
  • Cytotoxicity assays (lactic dehydrogenase release, trypan blue exclusion).

Related Experiment Videos

  • Beta-adrenergic receptor binding studies using radiopindolol.
  • Cell separation techniques (rosetting) to isolate B and T cells.

    • Main Results:

    • CLL lymphocytes aggregated in response to arachidonic acid, excess calcium, and epinephrine.
    • Epinephrine-induced aggregation was not cytotoxic and was blocked by propranolol.
    • Beta-adrenergic receptors were identified on CLL B cells, with a higher affinity (lower Kd) compared to T cells.
    • Receptor density was similar on normal and CLL B cells, and between B and T cells.

    Conclusions:

    • CLL B cells possess functional beta-adrenergic receptors.
    • Adrenergic stimulation can induce B cell aggregation, potentially altering lymphocyte traffic.
    • B cell aggregation may contribute to morbidity in rare CLL patients with extremely high lymphocyte counts.