A technique for the in vitro incubation of deer antler tissue

1. A procedure for the in vitro incubation of velvet deer antler tissue was developed. Biopsy samples were collected in June with a trephine from 2 adult white-tailed deer and incubated in modified BGJb medium up to 48 hr. Calcium (Ca) and hydroxyproline (OH-proline) concentrations in the tissue were determined. 2. A significant increase (P less than 0.05) in Ca was exhibited at 4 and 8 hr of incubation, and, after replenishment of media, at 48 hr. 3. Hydroxyproline concentrations continued to rise throughout the duration of the incubation period and were significantly higher than controls (P less than 0.05) at 16, 24, and 48 hr. 4. Results suggest antler tissue can be incubated in vitro with the protocol described, although length of incubation may vary with parameter measured.