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Mechanistic insight into the suppression of microglial ROS production by voltage-gated proton channels (VSOP/Hv1)

Takafumi Kawai1, Shoki Tatsumi1,2,3, Shinji Kihara3

  • 1a Integrative Physiology, Department of Physiology, Graduate School of Medicine , Osaka University , Osaka , JAPAN.

Channels (Austin, Tex.)
|September 30, 2017
PubMed

Insights

Voltage-gated proton channels (VSOP/Hv1) normally aid reactive oxygen species (ROS) production. However, VSOP/Hv1 deficiency unexpectedly increases ROS in microglia, suggesting unique cellular regulation.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Voltage-gated proton channels (VSOP/Hv1) are implicated in reactive oxygen species (ROS) generation in immune cells.
  • Previous studies suggest VSOP/Hv1 promotes ROS production.

Purpose of the Study:

  • To investigate the role of VSOP/Hv1 in ROS regulation specifically within microglia.
  • To explore the unique mechanisms governing VSOP/Hv1-dependent ROS production in microglia compared to other immune cells.

Main Methods:

  • Comparative analysis of ROS production in primary microglia from VSOP/Hv1-deficient and wild-type (WT) mice.
  • Assessment of ROS levels in microglia and macrophages under basal and lipopolysaccharide (LPS)-stimulated conditions.

Main Results:

  • Primary microglia from VSOP/Hv1-deficient mice exhibited higher ROS production than WT microglia.
  • This elevated ROS production in deficient microglia persisted even after LPS stimulation.
  • Macrophages did not show increased ROS production in the absence of VSOP/Hv1, indicating cell-specific regulation.

Conclusions:

  • Microglia display an unconventional VSOP/Hv1-dependent regulation of ROS production.
  • The findings suggest a unique cellular mechanism for ROS control in microglia that differs from macrophages.
  • Further research is needed to elucidate the specific pathways involved in this microglial VSOP/Hv1-mediated ROS regulation.
Keywords:
ROSVSOP/Hv1microgliamicroglial activationvoltage-gated proton channel

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