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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Related Experiment Video

Updated: Feb 22, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

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BSviewer: a genotype-preserving, nucleotide-level visualizer for bisulfite sequencing data.

Kun Sun1,2, Fiona F M Lun1,2, Peiyong Jiang1,2

  • 1Li Ka Shing Institute of Health Sciences.

Bioinformatics (Oxford, England)
|September 30, 2017
PubMed
Summary

BSviewer is a new bioinformatics tool that visualizes DNA methylation at the nucleotide level, aiding in the interpretation of allele-specific methylation patterns. This tool enhances understanding of bisulfite sequencing data beyond high-level views.

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Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Bisulfite sequencing is crucial for studying DNA methylation profiles across species.
  • Current visualization tools often lack nucleotide-level detail and omit important data like genotypes.
  • There is a need for tools that visualize methylation dynamics at the nucleotide level and retain essential sequencing information.

Purpose of the Study:

  • To develop a bioinformatics tool for visualizing bisulfite sequencing data at the nucleotide level.
  • To address the limitations of existing tools that provide only high-level methylation views.
  • To create a tool that preserves essential sequencing data, including genotypes on SNP sites, for better interpretation.

Main Methods:

  • Development of BSviewer, a lightweight visualization tool.
  • Implementation in Perl, compatible with GNU/Linux platforms.
  • Freely available source code and testing dataset.

Main Results:

  • BSviewer enables nucleotide-level visualization of bisulfite sequencing data.
  • Demonstrated utility in interpreting allele-specific DNA methylation patterns using an imprinting gene.
  • Provides a more detailed view compared to existing high-level methylation density tools.

Conclusions:

  • BSviewer offers a valuable solution for detailed analysis of bisulfite sequencing data.
  • The tool facilitates a deeper understanding of DNA methylation dynamics.
  • It is particularly useful for studies involving allele-specific methylation and genotype information.