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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

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Evaluation of expression levels and mechanism of complement activation

Xing Wang1, An-Heng Liu2, Zhong-Wei Jia1

  • 1Department of Cardiology, The 254th Hospital of PLA, Tianjin 300142, P.R. China.

Experimental and Therapeutic Medicine
|October 1, 2017
PubMed

Insights

Complement system activation and elevated C3a receptor expression occur in acute myocardial infarction (AMI). This suggests the C3a-C3a receptor pathway may contribute to heart attack development.

Area of Science:

  • Cardiovascular Medicine
  • Immunology
  • Molecular Biology

Background:

  • Acute myocardial infarction (AMI) involves complex inflammatory processes.
  • The role of the complement system in AMI pathogenesis requires further elucidation.

Purpose of the Study:

  • To investigate complement system activation during AMI.
  • To determine the expression levels of the C3a receptor in myocardial infarction tissues.

Main Methods:

  • Plasma levels of complement activation products (C3a, C4a, C5a) were measured using cytometric bead array.
  • C3a receptor protein expression in cardiac tissues was assessed via immunoblotting.
  • Study included healthy individuals and patients with coronary artery stenosis or AMI.

Main Results:

  • Significantly elevated levels of C3a, C4a, and C5a were observed in patients with coronary artery stenosis and AMI compared to controls (P<0.01).
  • Protein expression of the C3a receptor was significantly higher in infarct tissues from AMI patients than in adjacent normal tissues (P<0.05).

Conclusions:

  • Complement system activation is evident in patients with acute myocardial infarction.
  • Increased C3a receptor expression in infarct zones suggests the C3a-C3a receptor pathway's involvement in myocardial infarction development.

Keywords:
C3a receptoracute myocardial infarctioncomplement system

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