Effects of chromic chloride on chick embryo fibroblast viability

The objective of this study is to evaluate the effects of chromic chloride (CrCl₃) on chick embryo fibroblast (CEF) viability. The cells were incubated with CrCl₃ (0.02, 0.1, 0.5, 2.5, 12.5, and 62.5 μM), and the viability was determined using MTT assay, morphological detection and flow cytometry. The results show that lower concentrations of CrCl₃ (0.02, 0.1, and 0.5 μM) did not damage CEF viability. At 0.1 μM, CrCl₃ can increase CEF viability (P < 0.05). However, at higher concentrations of CrCl₃ (2.5, 12.5, and 62.5 μM), the number of apoptotic and necrotic cells (P < 0.01) and intracellular reactive oxygen species (P < 0.01) increased. In addition, decreased mitochondrial membrane potential (P < 0.01) and enhanced intracellular calcium levels (P < 0.01) were observed after the exposure. Moreover, apoptotic morphological changes induced by these processes in CEF were confirmed using Hoechst 33258 staining. Cell death induced by higher concentrations of CrCl₃ was caused by an apoptotic and a necrotic mechanism, whereas the main mechanism of oxidative stress and induced mitochondrial dysfunction was apoptotic death. The induced apoptotic death in CEF is concentration- and time-dependent.