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Evolutionary relationships in the genus Bordetella.

B Aricò1, R Gross, J Smida

  • 1Sclavo Research Centre, Siena, Italy.

Molecular Microbiology
|November 1, 1987
PubMed
Summary

Bordetella pertussis strains are genetically similar, distinct from B. parapertussis and B. bronchiseptica. This suggests B. pertussis does not evolve from B. parapertussis, challenging previous theories on whooping cough bacteria evolution.

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Area of Science:

  • Microbiology
  • Evolutionary Biology
  • Genetics

Background:

  • The pertussis toxin operon sequences of Bordetella species reveal common mutations, suggesting shared ancestry.
  • Understanding evolutionary relationships among Bordetella species is crucial for tracking whooping cough (pertussis) pathogen evolution.

Purpose of the Study:

  • To investigate the evolutionary relationships between Bordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica.
  • To analyze genetic variations in the pertussis toxin S1 subunit gene and its promoter region across different Bordetella strains.

Main Methods:

  • Cloning and sequencing of the promoter region and S1 subunit gene of pertussis toxin from various Bordetella pertussis strains.
  • Comparative sequence analysis of Bordetella species using computer-aided evolutionary tree construction.

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Main Results:

  • B. pertussis strains BP SA1 and BP 13456 showed minimal divergence (one base pair) from published sequences.
  • The type strain BP 18323 exhibited 11 base-pair substitutions, with 9 common to B. parapertussis and B. bronchiseptica.
  • Sequence data indicated B. pertussis strains are highly homogeneous and genetically distant from B. parapertussis and B. bronchiseptica.

Conclusions:

  • B. pertussis type strain BP 18323 likely shares a common ancestor with B. parapertussis and B. bronchiseptica.
  • The genetic homogeneity of B. pertussis strains supports their distinct evolutionary trajectory.
  • The proposed evolutionary conversion from B. parapertussis to B. pertussis is considered highly improbable based on genetic evidence.