Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Special Staining Techniques01:13

Special Staining Techniques

1.4K
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
1.4K
Differential Staining Technique01:26

Differential Staining Technique

2.5K
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
2.5K
Methods of Classification and Identification01:28

Methods of Classification and Identification

1.3K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.3K
Simple Staining Technique01:24

Simple Staining Technique

4.5K
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
4.5K
Fixation and Sectioning01:03

Fixation and Sectioning

8.2K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA Topoisomerase Iα Affects the Floral Transition.

Plant physiology·2016
Same author

Machine Learning Based Single-Frame Super-Resolution Processing for Lensless Blood Cell Counting.

Sensors (Basel, Switzerland)·2016
Same author

Autophagy in long propriospinal neurons is activated after spinal cord injury in adult rats.

Neuroscience letters·2016
Same author

Human Lysozyme Synergistically Enhances Bactericidal Dynamics and Lowers the Resistant Mutant Prevention Concentration for Metronidazole to Helicobacter pylori by Increasing Cell Permeability.

Molecules (Basel, Switzerland)·2016
Same author

Therapeutic effect of apatinib on overall survival is mediated by prolonged progression-free survival in advanced gastric cancer patients.

Oncotarget·2016
Same author

Prevalence of hemorrhagic fever with renal syndrome in Qingdao City, China, 2010-2014.

Scientific reports·2016
Same journal

[Research progress on multimodal precision nursing interventions in urinary incontinence rehabilitation management after radical prostatectomy].

Zhonghua nan ke xue = National journal of andrology·2025
Same journal

[Dietary intervention to improve erectile dysfunction: Progress in research].

Zhonghua nan ke xue = National journal of andrology·2025
Same journal

[Tonifying kidney and activating blood therapy for the treatment of diabetic erectile dysfunction: A systematic review and meta-analysis].

Zhonghua nan ke xue = National journal of andrology·2025
Same journal

[Clinical effect of Huotujiji prescription on patients with asthenospermia of spleen-kidney yang-deficiency type].

Zhonghua nan ke xue = National journal of andrology·2025
Same journal

[Application of predictive nursing to the rehabilitation of patients after endoscopic surgery for prostate under the concept of enhanced recovery after surgery].

Zhonghua nan ke xue = National journal of andrology·2025
Same journal

[Influencing factors of lower urinary tract symptoms in patients after radical prostatectomy and nursing strategy].

Zhonghua nan ke xue = National journal of andrology·2025
See all related articles

Related Experiment Video

Updated: Feb 22, 2026

Author Spotlight: A Simple and Cost-Effective Method for Leukocyte Analysis in Semen
04:36

Author Spotlight: A Simple and Cost-Effective Method for Leukocyte Analysis in Semen

Published on: January 19, 2024

5.6K

[Advanced technologies in semen stain identification].

Ying-Guo Wen1, Hao Yu2, Jun-Sheng Lin1

  • 1School of Biomedicine, Huaqiao University, Quanzhou, Fujian 362021, China.

Zhonghua Nan Ke Xue = National Journal of Andrology
|October 1, 2017
PubMed
Summary
This summary is machine-generated.

Forensic semen stain identification uses DNA and RNA for personalized evidence. New methods like spectroscopy and methylation assays improve accuracy over older protein-based techniques.

Keywords:
DNA methylationaptamermass spectrometryprotein markersemen stain identification

More Related Videos

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
09:17

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

Published on: April 12, 2018

18.1K
Fluorimetric Techniques for the Assessment of Sperm Membranes
08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

14.3K

Related Experiment Videos

Last Updated: Feb 22, 2026

Author Spotlight: A Simple and Cost-Effective Method for Leukocyte Analysis in Semen
04:36

Author Spotlight: A Simple and Cost-Effective Method for Leukocyte Analysis in Semen

Published on: January 19, 2024

5.6K
Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
09:17

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

Published on: April 12, 2018

18.1K
Fluorimetric Techniques for the Assessment of Sperm Membranes
08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

14.3K

Area of Science:

  • Forensic Science
  • Biochemistry

Background:

  • Semen stain identification is crucial for criminal evidence collection.
  • Conventional methods rely on protein markers (e.g., PSA), but face limitations like low sensitivity and stability.
  • Personalized evidence from semen stains (DNA, RNA) is vital for inferring crime details.

Purpose of the Study:

  • To review limitations of conventional semen stain identification methods.
  • To explore novel technologies for enhanced forensic trace analysis.
  • To highlight advancements in personalized and over-timed specimen detection.

Main Methods:

  • Review of emerging technologies including Raman spectroscopy, mass spectrometry, aptamers, mRNA, microRNA, and DNA methylation assays.
  • Comparison of new techniques against traditional protein-based forensic methods.
  • Focus on improved sensitivity, specificity, and stability for trace evidence analysis.

Main Results:

  • New technologies offer higher specificity and sensitivity compared to conventional protein markers.
  • Advanced methods show potential for detecting over-timed semen specimens.
  • Personalized trace analysis is significantly enhanced by these novel approaches.

Conclusions:

  • Emerging technologies overcome limitations of traditional semen stain identification methods.
  • Novel techniques provide more reliable and personalized forensic evidence.
  • Advancements pave the way for more effective criminal investigations.