Related Experiment Video
Updated: Feb 22, 2026
08:17
Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
Published on: July 9, 2021
5.3K
Parallel acquisition of slice-selective 1H-1H soft COSY spectra
Upendra Singh1, Ajay Verma1, Bikash Baishya2
1Centre of Biomedical Research (Formerly Centre of Biomedical Magnetic Resonance), SGPGIMS Campus, Raebareli Road, Lucknow 226014, India; Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|October 1, 2017
Summary
This study introduces a novel method for rapidly acquiring multiple slice-selective soft COSY proton spectra. This technique enhances efficiency by enabling selective coherence transfer, providing comprehensive coupling information for proton sites.
Area of Science:
- Magnetic Resonance Spectroscopy
- Nuclear Magnetic Resonance (NMR) Techniques
Background:
- Traditional COSY (Correlation Spectroscopy) experiments can be time-consuming.
- Acquiring detailed coupling information for all proton sites requires extensive data collection.
Purpose of the Study:
- To develop a method for parallel acquisition of slice-selective soft COSY proton spectra.
- To reduce experimental time while obtaining comprehensive spin-spin coupling data.
Main Methods:
- Implementation of a slice-selective mixing pulse within a selective correlation experiment.
- Utilizing slice-selective coherence transfer between coupled spin pairs.
- Ensuring passive spin states remain undisturbed during transfer.
Main Results:
- Demonstrated parallel acquisition of multiple slice-selective soft COSY spectra.
- Achieved slice-selective coherence transfer between coupled spins.
- Generated spectra containing multiple soft COSY spectra for a given signal and its coupled partners.
- Significantly reduced experimental time.
Conclusions:
- The developed method provides efficient access to all couplings for a given proton site and its coupled partners.
- This technique offers a substantial improvement in experimental speed for NMR spectroscopy.
Related Concept Videos
01:192D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
1.5K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.5K
01:062D NMR: Homonuclear Correlation Spectroscopy (COSY)
2.0K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
2.0K
01:02¹H NMR: Interpreting Distorted and Overlapping Signals
1.6K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.6K
01:162D NMR: Overview of Homonuclear Correlation Techniques
708
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
COSY90 is the standard two-dimensional (2D) COSY experiment that...
708
01:04¹³C NMR: ¹H–¹³C Decoupling
1.9K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.9K
01:13¹H NMR Signal Multiplicity: Splitting Patterns
6.9K
When protons A and X are coupled, their nuclear spin energy levels are slightly modified. This is because the energy required to excite proton A to a spin state parallel to proton X is slightly different from the energy required for it to become anti-parallel to spin X. Consequently, there are two possible excitation frequencies for A (A1 and A2), depending on the spin state of X, and vice versa. The mutual nature of coupling implies that the difference between frequencies A1 and A2, indicated...
6.9K