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Transamidating activities of factor XIIIa and of transglutaminases, measured by an ELISA procedure
P T Velasco1, F Karush, L Lorand
1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208.
Biochemical and Biophysical Research Communications
|April 29, 1988
Abstract:
The dansyl hapten in dansylcadaverine offers unique possibilities for measuring the incorporation of the monoamine into proteins (e.g. N,N'-dimethylcasein) by transamidating enzymes such as factor XIIIa and the transglutaminases. The protein-bound dansylcadaverine was assayed by an ELISA procedure based on a monoclonal antibody to the dansyl moiety.