Prostacyclin-inhibition of lysine accumulation by the rat left ventricle

1. Left ventricular slices of male Sprague-Dawley rats were incubated with a fixed concentration of 0.5 microCi/ml 3H-lysine and several concentrations of unlabelled lysine ranging from 0.2 to 5.0 mM in control and prostacyclin-treated experiments. The time of incubation ranged from 0.5 to 90 min. 2. Left ventricular slices were cut to have an optimal thickness of 0.47 +/- 0.09 mm. 3. Lysine was taken up against a concentration gradient. Saturation was reached at 0.5 mM and steady state accumulation of lysine was attained within 60 min. 4. Prostacyclin in concentrations ranging from 1.2 x 10(-8) to 4.8 x 10(-8) M inhibited lysine transport in left ventricular slices significantly (P less than 0.01).