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Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun

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Published on: October 24, 2014

Fast spatial-selective delivery into live cells.

Ranhua Xiong¹, Claire Drullion², Peter Verstraelen³

¹Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, Belgium; Centre for Nano- and Biophotonics, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, Belgium.

Journal of Controlled Release : Official Journal of the Controlled Release Society

|October 3, 2017

Summary

This summary is machine-generated.

We developed a new method called spatially resolved nanoparticle-enhanced photoporation (SNAP) for precise intracellular delivery of compounds into specific cells. This high-throughput technology enables targeted delivery to cell subpopulations or single cells for research and therapeutics.

Keywords:

Cell-selective delivery Intracellular delivery Nanoparticles Pulsed laser Vapour nanobubbles

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Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

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Published on: November 7, 2013

Area of Science:

Cell Biology
Biotechnology
Nanotechnology

Background:

Intracellular delivery of compounds is crucial for cell biology and therapeutics.
Targeted delivery to specific cell subpopulations or single cells remains a challenge.
Existing methods often lack precision and high-throughput capabilities for selective cell manipulation.

Purpose of the Study:

To develop an integrated platform for high-throughput, spatially resolved nanoparticle-enhanced photoporation (SNAP).
To enable safe and precise intracellular delivery of nanomaterials into selected cell subpopulations or single adherent cells.
To demonstrate the utility of SNAP for selective cell labeling and targeted drug delivery applications.

Main Methods:

Development of an integrated platform for spatially resolved nanoparticle-enhanced photoporation (SNAP).
Utilizing nanomaterials for enhanced photoporation in adherent cells.
High-throughput screening and selective targeting of cells based on spatial resolution.

Main Results:

SNAP enables safe, intracellular delivery of nanomaterials into selected cell subpopulations, including single cells.
Demonstrated selective delivery of a contrast agent into polynucleated keratinocytes for downstream purification.
The platform offers flexibility and speed for high-throughput, spatially resolved cell labeling.

Conclusions:

SNAP is a powerful tool for precise intracellular delivery in selected cell populations.
This technology facilitates downstream purification and functional studies of specific cell types.
SNAP holds significant potential for advancing cell biology research and targeted drug delivery strategies.