Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.7K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.7K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.7K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.9K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.9K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

9.2K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ensuring bladder volume reproducibility and reducing cone-beam computed tomography-related radiation exposure in prostate hypofractionated radiotherapy by a Certified Nurse in Radiation Oncology nursing.

Journal of radiation research·2026
Same author

[Pneumatosis cystoides intestinalis with extensive intraperitoneal free air, retroperitoneal emphysema, and pneumomediastinum:a case report].

Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology·2026
Same author

Clinical Outcomes of Hypofractionated Radiotherapy With 12 Fraction for Low- and Intermediate-Risk Localized Prostate Cancer.

International journal of urology : official journal of the Japanese Urological Association·2026
Same author

Successful treatment of Hori nevus with a 755 nm ps alexandrite laser with a diffractive lens array.

JAAD case reports·2025
Same author

Risk Factors for Cement Leakage in Cement-Augmented Pedicle Screw Fixation: A Multicenter Study of 140 Patients and 650 Screws.

Global spine journal·2025
Same author

Secukinumab Persistence in Patients with Psoriatic Arthritis: An Adalimumab-Matched Retrospective Cohort Database Study (FLYWAY).

Rheumatology and therapy·2025

Related Experiment Video

Updated: Feb 22, 2026

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

15.0K

Native mass spectrometry for understanding dynamic protein complex.

Kentaro Ishii1, Min Zhou2, Susumu Uchiyama3

  • 1Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan.

Biochimica Et Biophysica Acta. General Subjects
|October 3, 2017
PubMed
Summary

Native mass spectrometry reveals dynamic aspects of biomolecular complexes, overcoming limitations of traditional methods for studying large, heterogeneous assemblies in solution.

Keywords:
Electrospray ionizationIon mobility separationMembrane proteinNative mass spectrometryProtein-protein complexStoichiometry

More Related Videos

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

24.9K
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

20.5K

Related Experiment Videos

Last Updated: Feb 22, 2026

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

15.0K
Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

24.9K
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

20.5K

Area of Science:

  • Biophysics
  • Structural Biology
  • Biochemistry

Background:

  • Biomolecular complexes are crucial for cellular functions, but their study is challenging due to size and heterogeneity.
  • Traditional structural biology techniques face limitations with large, dynamic, and heterogeneous macromolecular assemblies.

Purpose of the Study:

  • To highlight the utility of native mass spectrometry in characterizing biomolecular complex dynamics.
  • To demonstrate how native mass spectrometry complements other biophysical methods.

Main Methods:

  • Native mass spectrometry (MS) was employed to analyze protein complexes.
  • Combined native MS with analytical ultracentrifugation, small-angle neutron scattering, and small-angle X-ray scattering.
  • Investigated soluble and membrane-embedded protein assemblies.

Main Results:

  • Native MS effectively probes dynamic processes like structural reorganization and subunit exchange.
  • Demonstrated the capability to study assembly and disassembly of complexes in solution.
  • Revealed insights into transient non-covalent interactions governing complex behavior.

Conclusions:

  • Native mass spectrometry is a powerful tool for understanding the dynamic nature of biomolecular complexes.
  • This technique overcomes limitations of traditional methods for studying large and heterogeneous assemblies.
  • Native MS, alone or combined with other methods, provides comprehensive insights into complex behavior in solution.