Related Experiment Video
Updated: Feb 22, 2026
A Modified Simple Method for Induction of Myocardial Infarction in Mice
Published on: December 3, 2021
FK506 protects heart function via increasing autophagy after myocardial infarction in mice
Yunle Wang1, Jia Lu2, Weili Cheng3
1Department of Cardiology, Wuxi People's Hospital, Wuxi, Jiangsu, China.
Objective:
To investigate the effect of tacrolimus (FK506) on myocardial infarction, and to further explore its function mechanism.
Material And Methods:
C57BL/6J mice were randomly divided into three groups: the sham group, the control group and the FK506 group. Anterior descending branch ligation was conducted in the control and the FK506 groups, while sham operation was conducted in the sham group. Mice in the sham and the control groups were intragastrical administration with saline, while the FK506 group were with FK506. Heart function were detected by echocardiogram at 3rd day or 21st day after MI. Hearts were harvested at 3rd day or 21st day after MI for the detection of apoptosis, autophagy, mTOR and NF-κB pathway.
Results:
FK506 treatment increased survival rate and cardiac function in mice after MI. It decreased infarction area, inflammation reaction and apoptosis. To further study the mechanism of FK506 protection effect, we discovered it could increase autophagy via inhibit mTOR pathway.
Conclusion:
FK506 protect heart function after MI as it improved myocardial cells autophagy process via inhibiting mTOR pathway.
More Related Videos
05:41Left Anterior Descending Coronary Artery Ligation for Ischemia-Reperfusion Research: Model Improvement via Technical Modifications and Quality Control
Published on: December 16, 2022
07:41Coronary Artery Ligation and Intramyocardial Injection in a Murine Model of Infarction
Published on: June 7, 2011