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Comparative proteomic analysis between two haemocyte subpopulations in shrimp Fenneropenaeus chinensis
Lei Zhu1, Yanhong Chang1, Jing Xing1
1Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao 266003, PR China.
Fish & Shellfish Immunology
|October 3, 2017
Summary
This study identifies distinct protein profiles of shrimp granulocytes and hyalinocytes using advanced proteomic techniques. These findings enhance understanding of immune cell functions in crustaceans.
Area of Science:
- Marine Biology
- Immunology
- Proteomics
Background:
- Shrimp immune cells, granulocytes and hyalinocytes, were previously separated using immunomagnetic bead (IMB) method.
- Understanding proteomic differences is crucial for elucidating immune cell functions.
Purpose of the Study:
- To analyze proteomic differentiation between shrimp granulocytes and hyalinocytes.
- To identify differentially expressed proteins in haemocyte subpopulations.
Main Methods:
- Two-dimensional gel electrophoresis (2-DE) combined with mass spectrometry (MS) for protein analysis.
- Immunomagnetic bead (IMB) separation of fixed/permeabilized (FP) haemocytes into granulocytes and hyalinocytes.
- Quantitative PCR (qPCR) for gene transcription level validation.
Main Results:
- Identified 4 differentially expressed proteins in non-fixed/un-permeabilized (NFP) haemocytes.
- Discovered 21 differentially expressed proteins between granulocytes and hyalinocytes.
- Found 4 unique proteins in granulocytes and 13 highly expressed proteins in hyalinocytes.
- Classified proteins into six functional categories including binding and catalytic activity.
- Confirmed proteomic trends with qPCR data for selected genes.
Conclusions:
- The study successfully elucidated proteomic differences between shrimp granulocytes and hyalinocytes.
- Identified proteins are involved in diverse cellular functions, aiding in understanding haemocyte subpopulation roles.
- Proteomic data aligns with gene transcription levels, validating the findings.
