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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Related Experiment Video

Updated: Feb 22, 2026

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
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Multi-MHz laser-scanning single-cell fluorescence microscopy by spatiotemporally encoded virtual source array.

Jianglai Wu1, Anson H L Tang1, Aaron T Y Mok1

  • 1Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China.

Biomedical Optics Express
|October 3, 2017
PubMed
Summary

We developed an ultrafast fluorescence microscopy technique using an all-optical laser-scanning mechanism. This method achieves high-throughput single-cell imaging and fast cellular dynamics, significantly improving imaging speed for biological research.

Keywords:
(110.0180) Microscopy(110.2970) Image detection systems(120.5800) Scanners(170.0110) Imaging systems(180.2520) Fluorescence microscopy

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Area of Science:

  • Biophysics
  • Optical Microscopy
  • Cell Biology

Background:

  • Scaling temporal resolution (imaging throughput) is crucial for fluorescence microscopy.
  • Existing imaging strategies face inherent speed limitations.
  • High-throughput and high-speed cellular imaging are vital for cell biology and diagnostics.

Purpose of the Study:

  • To address the speed limitations in fluorescence microscopy.
  • To demonstrate an ultrafast all-optical laser-scanning mechanism.
  • To enable high-throughput and high-speed biological imaging.

Main Methods:

  • Employing an all-optical laser-scanning mechanism with reconfigurable spatiotemporally-encoded virtual sources.
  • Achieving a line-scan rate as high as 8 MHz.
  • Demonstrating compatibility with existing imaging modalities.

Main Results:

  • Achieved high-throughput single-cell microfluidic fluorescence imaging at 75,000 cells/second.
  • Enabled high-speed cellular 2D dynamical imaging at 3,000 frames per second.
  • Outperformed state-of-the-art high-speed cameras and laser scanning strategies.

Conclusions:

  • The developed ultrafast fluorescence microscopy technique overcomes speed limitations.
  • This technology significantly enhances imaging throughput for biological applications.
  • It paves the way for new high-throughput and high-speed microscopy in cell biology and diagnostics.