Related Experiment Video

Updated: Feb 22, 2026

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
11:22

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins

Published on: October 29, 2013

43.7K

Producing GST-Cbx7 Fusion Proteins from Escherichia coli

Thao Ngoc Huynh1, Xiaojun Ren1

  • 1Department of Chemistry, University of Colorado Denver, Denver, CO, USA.

Bio-Protocol
|October 3, 2017
PubMed

Keywords:
Affinity purificationCbx7GST-fusion proteinsGlutathione Sepharose 4BPolycombpGEX-6P-1

More Related Videos

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology16:11

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Published on: September 16, 2013

66.4K
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

13.9K

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
8.6K