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Related Experiment Video

Updated: Feb 22, 2026

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Solid-State Nanopore Analysis of Diverse DNA Base Modifications Using a Modular Enzymatic Labeling Process.

Fanny Wang¹, Osama K Zahid¹, Brandi E Swain²

¹Department of Biomedical Engineering, Virginia Tech-Wake Forest University, School of Biomedical Engineering and Sciences, Wake Forest School of Medicine , Winston-Salem, North Carolina 27101, United States.

|October 3, 2017

Summary

This study introduces a novel method for detecting various modified DNA bases using enzymatic labeling and nanopore technology. This approach enables precise identification of DNA base modifications, crucial for understanding gene expression and disease.

Keywords:

Nanopore base lesion cancer detection epigenetics

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Last Updated: Feb 22, 2026

Nanopore DNA Sequencing for Metagenomic Soil Analysis

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Published on: December 14, 2017

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Published on: August 26, 2009

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Epigenetic elements and DNA base lesions impact gene expression and disease.
Conventional technologies struggle to assess noncanonical DNA modifications.

Purpose of the Study:

To develop a new approach for targeted detection of diverse modified bases in DNA.
To demonstrate the potential for quantitative nanopore assessment of base modifications.

Main Methods:

Utilized enzymatic components of the DNA base excision repair pathway for affinity labeling of modified bases.
Employed a solid-state nanopore assay to discriminate labeled from unlabeled DNA.
Demonstrated modularity by detecting uracil, 8-oxoguanine, T:G mismatch, and 1,N6-ethenoadenine.

Main Results:

Successfully installed affinity labels at specific modified DNA base locations with high yield.
The nanopore assay effectively discriminated between labeled and unlabeled DNA.
The technique demonstrated versatility in detecting multiple types of DNA base modifications.

Conclusions:

The developed method offers a powerful tool for targeted detection of diverse DNA base modifications.
This approach has the potential for quantitative nanopore assessment of a broad range of base modifications.
The technique's modularity allows for adaptation to various DNA base analyses.