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Prevalence of COMT Val158Met polymorphism in Eastern UP population
1Human Molecular Genetics Laboratory, Department of Biotechnology, VBS Purvanchal University, Jaunpur-222003, Uttar Pradesh, India.
Cellular and Molecular Biology (Noisy-Le-Grand, France)
|October 3, 2017
Summary
The Catechol-O-methyltransferase (COMT) Val158Met polymorphism was analyzed in 100 healthy individuals from the Scheduled Caste population in Jaunpur. This study determined the genotype and allele frequencies of this common COMT gene variant.
Area of Science:
- Genetics
- Biochemistry
- Population Studies
Background:
- Catechol-O-methyltransferase (COMT) is a key enzyme in catecholamine metabolism.
- The COMT gene's Val158Met polymorphism influences enzyme activity and is linked to various diseases.
- COMT activity impacts homocysteine levels through its role in S-adenosylmethionine (SAM) metabolism.
Purpose of the Study:
- To investigate the frequency of the COMT Val158Met polymorphism in the Scheduled Caste (SC) population of Jaunpur district.
- To provide baseline genetic data for this specific demographic group.
Main Methods:
- A total of 100 healthy, unrelated SC subjects from Jaunpur were recruited.
- Blood samples were collected for DNA extraction and analysis.
- Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was used to determine COMT genotypes.
Main Results:
- The genotype frequencies were: Val/Val (48%), Val/Met (40%), and Met/Met (12%).
- The allele frequencies were calculated as Val (68%) and Met (32%).
Conclusions:
- The study established the genotype and allele frequencies of the COMT Val158Met polymorphism in the SC population of Jaunpur.
- These findings contribute to understanding genetic diversity and may inform future health-related research in this population.
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