Related Experiment Video
Updated: Feb 21, 2026
Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
Published on: September 29, 2015
An Attempt to Make Platelet Rich Fibrin from Bone Marrow Aspirate
Soh Nishimoto1, Kazutoshi Fujita2, Toshihiro Fujiwara3
1Professor, Department of Plastic Surgery, Hyogo College of Medicine Nishinomiya, Hyogo, Japan.
Introduction:
Platelet Rich Fibrin (PRF) was developed as a new generation of platelet concentration from peripheral blood. Method to make PRF is simple. It is easy to handle with its moderate firmness. Histologically, platelets and nucleated cells are packed along the yellow-red border. Bone marrow aspirate contains bone marrow cells that potentially work for tissue regeneration, and platelets which contain growth factors. The specific gravities of them are comparable. It implies that, if it is possible to make PRF from bone marrow aspirate, then high concentration of platelets and bone marrow cells can be obtained simultaneously by taking out yellow-red interface of it.
Aim:
To find out a method to make PRF from bone marrow aspirate.
Materials And Methods:
Iliac crest of rabbits were punctured and aspirated with or without anti-coagulant, under general anaesthesia. The bone marrow aspirate was centrifuged in glass tubes. For the bone marrow aspirate taken with anti-coagulant, calcium chloride was added just before centrifugation. Products were taken out and observed grossly. The products were fixed with formaldehyde and observed histologically.
Results:
Coagulated gels with two-toned colour were obtained by all methods. In the gels without anti-coagulant, interfaces between two colours were obscure. Histologically, platelets and nucleated cells scattered as clusters. Filtering caused haemolysis and reduced the yield of the product. With the aspirate taken with anti-coagulant, platelets and nucleated cells formed a band along the interface.
Conclusion:
PRF can be made from bone marrow aspirate by adding anti-coagulant in aspiration and reversed with calcium chloride just before centrifugation.
Insights
Platelet Rich Fibrin (PRF) can be successfully prepared from bone marrow aspirate. This method yields a high concentration of platelets and bone marrow cells for tissue regeneration.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
Background:
- Platelet Rich Fibrin (PRF) is a second-generation platelet concentrate.
- PRF preparation is simple and yields a product with moderate firmness.
- Bone marrow aspirate contains regenerative cells and growth factor-rich platelets.
Purpose of the Study:
- To develop a method for preparing PRF from bone marrow aspirate.
- To achieve a simultaneous high concentration of platelets and bone marrow cells.
Main Methods:
- Rabbit iliac crest bone marrow was aspirated with or without anticoagulant.
- Aspirate was centrifuged in glass tubes, with calcium chloride added to anticoagulated samples before centrifugation.
- Products were grossly and histologically examined.
Main Results:
- All methods produced two-toned coagulated gels.
- Aspirate with anticoagulant and calcium chloride resulted in a distinct band of platelets and nucleated cells at the interface.
- Aspirate without anticoagulant showed scattered cells and obscure interfaces.
Conclusions:
- PRF can be prepared from bone marrow aspirate.
- Anticoagulant in aspiration and calcium chloride before centrifugation are key to obtaining concentrated platelets and cells.
