Quadriceps Muscles O₂ Extraction and EMG Breakpoints during a Ramp Incremental Test

Muscle deoxygenated breakpoint ([HHb]BP) has been found to be associated with other indices of exercise tolerance in the vastus lateralis (VL) muscle but not in the vastus medialis (VM) and rectus femoris (RF). Purpose: To investigate whether the [HHb]BP occurs also in the VM and RF muscles and whether or not it is associated with other physiological indices of exercise tolerance, such as the EMG threshold (EMG_t) and the respiratory compensation point (RCP). Methods: Twelve young endurance trained participants performed maximal ramp incremental (RI) cycling tests (25-30 W·min^-1 increments). Muscle oxygen extraction and activity as well as ventilatory and gas exchange parameters were measured. After accounting for the mean response time, the oxygen uptake ([Formula: see text]O₂) corresponding to the RCP, [HHb]BP, and the EMG_t was determined. Results: Peak power output (PO_peak) was 359 ± 48 W. Maximal oxygen consumption ([Formula: see text]O_2max) was 3.87 ± 0.46 L·min^-1. The [Formula: see text]O₂ at the RCP was 3.39 ± 0.41 L·min^-1. The [Formula: see text]O₂ (L·min^-1) corresponding to the [HHb]BP and EMG_t were: 3.49 ± 0.46 and 3.40 ± 0.44; 3.44 ± 0.61 and 3.43 ± 0.49; 3.59 ± 0.52, and 3.48 ± 0.46 for VL, VM, and RF, respectively. Pearson's correlation between these thresholds ranged from 0.90 to 0.97 (P < 0.05). No difference was found for the absolute [Formula: see text]O₂ and the normalized PO (%) at which the thresholds occurred in all three muscles investigated (P > 0.05). Although in eight out of 12 participants, the [HHb]BP in the RF led to a steeper increase instead of leading to a plateau-like response as observed in the VL and VM, the [Formula: see text]O₂ at the breakpoints still coincided with that at the RCP. Conclusions: This study demonstrated that local indices of exercise tolerance derived from different portions of the quadriceps are not different to the systemic index of the RCP.