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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Feb 21, 2026

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Scipion web tools: Easy to use cryo-EM image processing over the web.

Pablo Conesa Mingo¹, José Gutierrez¹, Adrián Quintana¹

¹Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, Madrid, 28049, Spain.

Protein Science : a Publication of the Protein Society

|October 4, 2017

Summary

Scipion Web Tools simplifies cryo-electron microscopy (cryo-EM) data processing for structural biology. This web-based framework makes complex image analysis accessible to nonexpert users, aiding macromolecular structure determination.

Keywords:

cryo-electron microscopy validation visualization web tools workflow

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Last Updated: Feb 21, 2026

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Cryo-EM and Single-Particle Analysis with Scipion

Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Published on: December 1, 2023

Area of Science:

Structural Biology
Biophysics
Computational Biology

Background:

Cryo-electron microscopy (cryo-EM) is a powerful technique for determining macromolecular structures.
The image processing pipeline for cryo-EM is complex and requires specialized software.
A need exists for user-friendly tools to assist nonexpert users in cryo-EM data analysis.

Purpose of the Study:

To present Scipion Web Tools, a web-based platform simplifying cryo-EM image processing.
To provide nonexpert users with precise answers at key stages of structural elucidation.
To make advanced cryo-EM data analysis more accessible.

Main Methods:

Development of a web-based interface for the Scipion image processing framework.
Integration of various image processing workflows tailored for cryo-EM.
Focus on usability for users with limited expertise in software installation and operation.

Main Results:

Scipion Web Tools offers a streamlined approach to cryo-EM data processing.
The platform facilitates precise answers for users at critical steps of structural determination.
Web-based accessibility reduces the barrier to entry for cryo-EM analysis.

Conclusions:

Scipion Web Tools enhances accessibility to cryo-EM for a broader scientific community.
The tools empower nonexpert users to achieve high-resolution structural information.
This framework supports the advancement of structural biology through simplified cryo-EM data processing.